Liquid Extraction: Folch | SpringerLink
The basic idea behind this extraction procedure was to homogenize a tissue sample (Folch and Lees 1951; Sperry and Brand 1955) in a mixture of chloroform and methanol in volumetric ratios of 2:1. The initial extraction step results in non-extractable residue and one liquid phase containing a wide range of small biomolecules.
